Project

Part:BBa_I3521:Design

Designed by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)   Group: Antiquity   (2004-01-28)


Test: Quorum threshold level and chemotaxis response, sender cells (I3600.I3520.I3500)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1222
    Illegal BglII site found at 1671
    Illegal BglII site found at 3136
    Illegal BglII site found at 6455
    Illegal BamHI site found at 5332
    Illegal XhoI site found at 5383
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2563
    Illegal AgeI site found at 2092
    Illegal AgeI site found at 2944
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4073
    Illegal BsaI.rc site found at 5176
    Illegal BsaI.rc site found at 5735
    Illegal SapI site found at 2603


Design Notes

Sender cells are the nonchemotactic E. coli strain RP5232. Receiver cells are wildtype E. coli. This test combines the chemotaxis response test with the quorum threshold test.



Source

References